Regulatory

Part:BBa_K5154000:Design

Designed by: Linhan Lei, Jürgen Heinisch   Group: iGEM24_Cambridge   (2024-09-27)


GALp-lexAops


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 377
    Illegal XbaI site found at 424
    Illegal PstI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 350
    Illegal PstI site found at 462
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 350
    Illegal BamHI site found at 371
    Illegal BamHI site found at 418
    Illegal XhoI site found at 318
    Illegal XhoI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 377
    Illegal XbaI site found at 424
    Illegal PstI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 377
    Illegal XbaI site found at 424
    Illegal PstI site found at 462
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

lexA comes from E.coli and is involved in the regulation of SOS responser, and Rlm1 is a native transcription factor in S. cerevisiae.

References

Straede, Andrea, et al. "The effect of tea tree oil and antifungal agents on a reporter for yeast cell integrity signalling." Yeast 24.4 (2007): 321-334.